The mission of the Nuclear Magnetic Resonance (NMR) technical platform is to offer researchers tools and expertise in NMR for the structural analysis of natural and man-made molecules and biological macromolecules, and for metabolomics, applied to the study of biodiversity and ecosystems. It has two NMR Bruker spectrometers, one at 400 MHz for routine use, the other at 600 MHz equiped with a cryoprobe and an automatic sample changer to be able to develop environmental metabolomic analysis and meet the requirements of as many establishment researchers as possible.


The NMR enables signals to be obtained which characterise the environment of certain atoms (in particular 1H, 13C, 31P and 15N). Used on purified molecules, it elucidates the flat and three-dimensional structures of molecules. On extracts with complex compositions, it provides characteristic metabolite profiles which enable classification, hence its essential application in metabolomic analysis.

This platform is incorporated in the UMR 7245 Communication Molecules and Adaptation of Micro-organisms (CNRS/MNHN).

Activities

  • Structural analysis of natural substances and their synthetic derivatives of various chemical structures, small molecules (polyphenols, alkaloids, terpenes, etc.) and of biological macromolecules (oligosaccharides, peptides).
  • Analysis and control of molecules entering a collection in all of the "Biological Resources" of the Muséum’s Collections (Muséum’s Chemical Library, the CNRS National Chemical Library).
  • Analyses of the metabolic profiles of organisms and micro-organisms.

The operation of the platform is coordinated by a scientific steering committee and subject to a charter of use.

Instruments

  • Bruker Avance III HD 400 MHz spectrometer, 9.4 Tesla shielded superconducting magnet, multinuclear BBFO Plus Smartprobe (1H, 13C, 19F, 31P, 15N), 16-tube sample changer.
  • Bruker Avance III HD 600 MHz spectrometer, 14.1 Tesla shielded superconducting magnet, triple resonance cryogenic probe (1H, 13C, 15N), 60-tube sample changer.
  • The technical platform also has computer systems which can do the calculations for molecular modelling under NMR constraints to determine three dimensional structures of biological macromolecules.

Platform website

Contacts

Séverine Zirah, coordinator
severine.zirah@mnhn.fr
Tel. +33(0)1 40 79 31 40

Benjamin Marie, coordinator
bmarie@mnhn.fr
Tel. +33(0)1 40 79 32 12

Alain Blond, technical manager
alain.blond@mnhn.fr
Tel. +33(0)1 40 79 31 31

Alexandre Deville
alexandre.deville@mnhn.fr
Tel. +33(0)1 40 79 31 31

Physical address:
Muséum national d’Histoire naturelle
Département Régulation Diversité Moléculaire
UMR 7245 Molécules de Communication et Adaptation des Microorganismes (CNRS/MNHN)
63, rue Buffon - CP 54
75005 Paris