The Muséum national d’Histoire naturelle’s electron microscopy and microanalysis technical platform is a service shared by all the Muséum’s scientists, but also available to public and private external users. This service is particularly dedicated to samples from the environment, from the mineral to the living. The instruments available, a Scanning Electron Microscope (SEM) and a Transmission Electron Microscope (TEM), enable, on the one hand, the study of the ultrastructure of surfaces in order to specify the structural, taxinomic and physiological characters, and on the other hand, using ultra-thin slices, a better understanding of the internal organisation of cells and their metabolism. These two types of equipment are complementary.


The two microscopes are in different premises (12 and 45 rue Buffon, Paris 5th arrondissement), but they are close enough to facilitate their shared management. They are each equipped with a digital image capture system. Staff are assigned to each apparatus and are in charge of routine maintenance as well as its operation and image acquisition.
This technical platform is incorporated in the UMR 7245 Communication Molecules and Adaptation of Micro-organisms (CNRS/MNHN)

Equipment

S.E.M.:

  • a JEOL 840 A microscope, equipped with an image-digitising system (SEMAFORE software);
  • a Hitachi SU 3500 microscope, equipped with an EDX detector for microanalysis (NSS software);
  • an EMITECH K 850 critical point dryer
  • a Jeol FJC-1200 metallizer

T.E.M.:

  • a HITACHI H-7100 microscope equipped with an image-digitising system (HAMAMATSU CCD camera linked to a computer with AMT image acquisition and processing software)
  • five ultramicrotomes (1 LKB 8800 4 Reichert-Jung ultracut E) + 3 Knife Maker LKB

Platform website (in French)

Contacts

Cécile Bernard, coordinator
cbernard@mnhn.fr

M.E.B. :
Catherine Rausch, scientific and technical manager
catherine.rausch-de-traubenberg@mnhn.fr
Tel. +33(0)1 40 79 31 91

Cyril Willig
cwillig@mnhn.fr
Tél : 01 40 79 36 91

Physical address:
Muséum national d’Histoire naturelle
Département Systématique et Evolution
UMR 7205 Origine, Structure et Evolution de la Biodiversité
Plateau technique de microscopie électronique et de microanalyse
45 rue Buffon - CP 39
75005 Paris
 

M.E.T. :
Chakib Djediat, scientific and technical manager
chakib.djediat@mnhn.fr
Tel. +33(0)1 40 79 31 85

Physical address:
Muséum national d’Histoire naturelle
Département Régulation, Développement, Diversité Moléculaire
UMR 7245 Molécules de Communication et Adaptation des Microorganismes (CNRS/MNHN)
Team Cyanobactéries, Cyanotoxines et Environnement
12 rue Buffon
75005 Paris